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Fig. 4 | Cell & Bioscience

Fig. 4

From: Stromal Senp1 promotes mouse early folliculogenesis by regulating BMP4 expression

Fig. 4

SENP1-deficient stromal cells are disorganized with reduced expression of BMP4. a SENP1-deficient stromal cells are disorganized. Stromal cell marker SM22α immunofluorescence in ovaries of WT and SENP1-smKO mice at D0, D3 and D7. SM22α+ stromal cells gradually mature to tightly wrap advanced follicles In WT ovaries. However, SENP1-smKO stromal cells exhibit delayed maturation and loosely surround follicles. Scale bars 15 μm. OC oocyte, SC stromal cell. b SENP1 and SM22α co-immunofluorescence staining of ovaries from WT and SENP-smKO mice. c Immunofluorescence staining of sm22α and phalloidin of human primary ovarian stromal cells. Scale bars 10 μm. d, e SM22α+ stromal cells were transfected with control siRNA or SENP1 siRNA. d SENP1, BMP2, BMP4 and BMP6 mRNAs were determined by qRT-PCR. Normalized mRNA levels (with GADPH) are presented as fold changes by taking control siRNA group as 1.0. e Protein level of BMP4 was determined by western blot with respective antibodies. Normalized protein levels (with GADPH) are presented as fold changes by taking control siRNA group as 1.0. Three independent experiments were performed

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