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Fig. 5 | Cell & Bioscience

Fig. 5

From: ZNF300 tight self-regulation and functioning through DNA methylation and histone acetylation

Fig. 5

The ZNF300 alters histone 3 lysine 9 acetylation. a, b Control (Ctrl), ZNF300 knockdown (shZNF300#2 and 4), or ZNF300 overexpression K562 cells were stimulated with vehicle or PMA and harvested for Western blot to measure H3K9 acetylation (H3K9Ac) and 3-methyl-H3K9 (H3K9me3) (left panel). Histone3 (H3) serves as loading control. All data are representative blots from two independent experiments with similar results and right panel was the average densitometry of the Western blot relative to the control. c Control (Ctrl) and ZNF300 overexpression K562 cells treated with PMA were harvested for ChIP assay with anti-H3K9Ac antibody. The DNA amount of ZNF300 gene promoter (indicated as R1, R2, R3, R4 in the top panel), ZNF300 zinc finger domain-encoding region (indicated as ZF in the top panel), and GAPDH gene promoter was quantified, normalized, and presented as relative enrichment. GAPDH serves as a negative control. Top schematic illustration shows the DNA regions used for quantitative PCR. Numbers indicate the nucleotide position relative to transcription start site. Data (mean ± STDEV) are statistics of one representative results (duplicates) from two independent experiments with similar results

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