Fig. 1

Zinc finger domain-encoding regions of KRAB-ZFPs mediate gene suppression. a Schematic illustration of reporter vectors shows SV40 promoter, the firefly luciferase, polyA element, and zinc finger domain-encoding regions (ZFR) of different zinc finger proteins. b 293T cells were transfected with firefly luciferase reporter vectors pGL3-promoter plasmids with different insertions as indicated. Luciferase activities were measured and presented as relative luciferase activity. c The mRNA level of firefly luciferase in the transfected 293T cells was measured by quantitative RT-PCR and represented as relative mRNA level. d 293T cells were transfected with luciferase reporter vectors driven by different promoters with (ZNF300ZFR) or without (NO ZNF300ZFR) zinc finger domain-encoding region of ZNF300 gene on the downstream as indicated. The luciferase activities were measured, normalized, and presented as relative luciferase activity. *, **, and *** indicate significance compared to control (p < 0.05, p < 0.01, and p < 0.001, respectively). All values are statistics of three independent experiments with triplicates and presented as mean ± STDEV