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Fig. 4 | Cell & Bioscience

Fig. 4

From: Epigenetic regulation of Runx2 transcription and osteoblast differentiation by nicotinamide phosphoribosyltransferase

Fig. 4

Regulative effects of Nampt on Runx2 transcription in MC3T3-E1 cell differentiation. MC3T3 cells were differentiated for 48 h and then co-transfected with pGL4.10-mRunX2pro firefly luciferase reporter (100 ng), pGL4.75 Renilla reporter (4 ng) and either 100 µM of scrambled RNA or Nampt shRNA using Lipofectamine 3000. Transfected MC3T3 cells were incubated for 24 h and luciferase activity was determined using Promega’s Dual-Glo Luciferase Assay Kit. Background corrected firefly luminescence values were normalized with Renilla luminescence values. Relative luciferase activities were normalized against differentiated MC3T3 cells transfected with pGL4.10 empty vector. n = 4; Bars are mean ± SD. **p < 0.01

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