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Fig. 1 | Cell & Bioscience

Fig. 1

From: Epigenetic regulation of Runx2 transcription and osteoblast differentiation by nicotinamide phosphoribosyltransferase

Fig. 1

Osteoblast differentiation of bone marrow stromal cells in wild type (Nampt +/+) and Nampt +/− mice. Cells were isolated and cultured in 12-well plate in RPMI for 10 days and then were differentiated in OBM for 14 days. a Western blot analysis of Nampt expression of differentiated osteoblast in Nampt +/+ and Nampt +/− mice. b Representative images of ALP stained differentiated osteoblasts. c ALP activity analyzed by PNPP quantification described in “Methods”. d Representative images of the differentiated osteoblast stained by Alizarin Red S. e Alizarin Red S stained differentiated osteoblast were counted using the Image J in three different view fields and the percentage of positive cells over total cells were calculated. n = 3, *p < 0.05 vs wild type mice

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