Fig. 3

UTX promotes thymic NKT maturation at the P1–P2 stage. a Thymocytes were isolated from female WT, JMJD3 KO, UTX KO, and UTX/JMJD3 KO mice. Cells were stained with CD1d tetramer reagent and other markers of NKT precursors. Plots were gated by light scatter on lymphocytes (left panels) or tetramer⁺ cells (right panels). Results are representative of 3 or more mice per group in 3 experiments. b Tetramer⁺ thymocytes were selected by magnetic enrichment and stained for CD69 and CD24. This plot shows that NKT P0 is intact and that these cells express CD69 in DKO mice. This stain was done on 3 WT and 3 DKO mice