Fig. 1

Loss of UTX and JMJD3 partially inhibits CD4 development, but CD8 T cells are intact. a Representative staining of splenocytes from WT, JMJD3, UTX and UTX/JMJD3 DKO mice were isolated and stained with the indicated markers of conventional T cells. Numbers of animals are indicated in d, e, and were analyzed in 3 separate experiments. b, c Cell counts from the spleens and thymi of WT and age matched litter mate control mice. Although cell counts vary across age, there is no reproducible difference between the genotypes in a given experiment. d, e CD4 and CD8 frequencies in the spleens of mice from the indicated genotypes. There is no significant difference between WT and UTX KO CD4 cells while JMJD3 and UTX/JMJD3 show statistically reduced CD4 frequency (two-tailed T test, p < 0.05). CD8 T cell frequency is unaffected by loss of these genes