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Table 1 Comparison of TALEN and CRISPR-Cas9 systems

From: Plant genome editing with TALEN and CRISPR

TALEN
Advantages Disadvantages
~30 bp target requirement results in less off-target effects Difficult protein engineering potentially increases time and financial investment
No PAM requirement; can target any sequence Efficiency varies for each construct
Cannot target methylated DNA
Difficult to engineer nickase
CRISPR-Cas9
Advantages Disadvantages
Able to multiplex Higher potential for off-target effects
Easy to engineer PAM requirement limits target
Can target methylated DNA
Easy to create a nickase