Fig. 3
NHEJ based genome editing applications. a NHEJ repair of an SSN induced break can create a premature stop codon. A stop codon is indicated by a red octagon. GOI is an acronym for gene of interest. b Non-protein coding genes such as microRNA and long non-coding RNA can be rendered non-functional through targeted mutations by SSNs. c Regulatory elements involved in the activation or repression of genes can be disrupted by SSNs. d Pieces of chromosomes that may involve regulatory networks or related genes can be deleted by SSNs