Skip to main content

Advertisement

Fig. 5 | Cell & Bioscience

Fig. 5

From: Upregulation of heme oxygenase-1 expression by curcumin conferring protection from hydrogen peroxide-induced apoptosis in H9c2 cardiomyoblasts

Fig. 5

Influence of LY294002 on apoptosis and HO-1 expression. a, b After pretreated with 15 μM of curcumin for 12 h in the absence or presence of 50 μM of LY294002, the cells were washed and incubated with 400 μM H2O2 for 3 h. c Cells were treated with 15 μM curcumin for 30 min in the absence or presence of 50 μM LY294002, which was added 1 h before curcumin. d Cells were incubated with 15 μM of curcumin for 12 h in the absence or presence of 50 μM of LY294002. The CC3, Bcl-2, Bax, HO-1, p-Akt and t-Akt protein expression were determined by western blot analysis. The Bcl-2/Bax ratio was calculated. Data were presented as mean ± SD (n = 3). *p < 0.05 vs. control, # p < 0.05 vs. H2O2, p < 0.05 vs. H2O2 + curcumin

Back to article page