Fig. 4

Curcumin enhanced phosphorylation of Akt but had no influence on ERK1/2 phosphorylation. Cells were treated with 15 μM curcumin for the indicated times. The expression levels of p-Akt, t-Akt, p-ERK1/2 and t-ERK1/2 were measured by western blot analysis. Cell extracts from H9c2 cells stimulated by osmotic shock (0.5 M sorbitol, 30 min) served as a positive control. Representative blots of three independent experiments were shown