Fig. 3
From: Large is required for normal astrocyte migration and retinal vasculature development
Persistent fatal vasculature (PFV) in Large PB/PB mutants. a–d Histological analysis of 2-month old wild-type and Large PB/PB mutants revealed remnant of hyaloid vessels (b, d, arrow) and ectopic cells anterior to inner limiting membrane (d, arrowhead) in the mutant mice. e Power Doppler mode of ultrasonic imaging for the eyes of 2-month old wild-type mice. No blood flow was observed around the posterior surface of the lens (arrow). f Power Doppler mode of ultrasonic imaging detected blood flow (arrowhead) in the persistent hyaloid vessels of 2-month old Large PB/PB mice. g Electron microscopy did not show any blood vessel in the vitreous of 2-month old wild-type mice. h Hematocytes (arrowhead) were observed in the persistent hyaloid vessels of 2-month old Large mutants under electron microscopy. Scale bars (a–d), 100 μm; scale bar (e, f), 1 mm; scale bar (g), 2 μm; scale bar (h), 20 μm