Fig. 1

LSD1 directly interacts with PLK1. a PLK1 inhibitor treatment reduced thymidine-nocodazole-induced LSD1 mobility shift in HeLa cells. HeLa cells were synchronized at metaphase by single thymidine block followed by nocodazole treatment for 12 h. Kinase inhibitors were added 2 h before total cell lysates were harvested. A dash line indicates the center of the shifted band of LSD1. Relative ratio of the unshifted band intensity of LSD1/β-actin was presented. Asy: asynchronized cells; syn: synchronized cells; long exp.: long exposure. b Endogenous LSD1 interacts with PLK1. Total cell lysates from 293T cells were subjected to immunoprecipitation followed by immunoblotting with antibodies as indicated. c Ectopic-tagged LSD1 interacts with PLK1. FLAG-PLK1 and HA-LSD1 were co-expressed in 293T cells. Total cell lysates were harvested 48 h after transfection and subjected to immunoprecipitation followed by immunoblotting with anti-HA or anti-FLAG antibodies. d LSD1 directly interacts with PLK1. Bacterially produced GST-PLK1 was used to pull down bacterially produced HIS-LSD1. Asterisk non-specific signal