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Fig. 8 | Cell & Bioscience

Fig. 8

From: Hsa-let-7c-5p augments enterovirus 71 replication through viral subversion of cell signaling in rhabdomyosarcoma cells

Fig. 8

Impact of the JNK inhibitor SP600125 on EV71 replication. a Effect of SP600125 on the CPE of EV71-infected RD cells. RD cells were pretreated with or without SP600125 for 1 h and infected with EV71 (MOI = 5) for 12, 24 or 36 h. Cell morphology was examined by light microscopy (40×). The CPE is labeled with red arrows. The scale bar is 100 μm, as shown in the first image. b Regulation of the virus titer by SP600125 at 24 h p.i. was assessed by plaque assays. The virus titers in the cultures are expressed as PFU per milliliter. c Regulation of the expression of EV71 RNA by SP600125 at 24 h p.i. was examined by qRT-PCR. d The effects of SP600125 on the VP1, total JNK1/2 and p-JNK1/2 protein levels were assessed by Western blotting. RD cells were pretreated with or without SP600125 for 1 h prior to EV71 infection (MOI = 5) or mock infection for 24 h. **p < 0.01; ***p < 0.001

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