Skip to main content

Advertisement

Fig. 2 | Cell & Bioscience

Fig. 2

From: Hsa-let-7c-5p augments enterovirus 71 replication through viral subversion of cell signaling in rhabdomyosarcoma cells

Fig. 2

EV71 replication is regulated by the hsa-let-7c-5p mimic. a The efficiency of transfection of the hsa-let-7c-5p mimic (100 nM) into RD cells for 48 h was determined by stem-loop qRT-PCR. b The EV71 titer is altered by the hsa-let-7c-5p mimic. RD cells were transfected with the NC (100 nM), hsa-let-7c-5p (100 nM) or neither (mock transfection, Mock-T) for 48 h, followed by EV71 infection (MOI = 5) for 24 h. Total virus was collected from infected cells and culture supernatants and used in plaque assays. The virus titers in the cultures are expressed as plaque-forming units (PFU) per milliliter. c The expression of viral RNA and (d) the viral structural protein VP1 was assessed by qRT-PCR and Western blotting, respectively, after transfection of cells with the hsa-let-7c-5p mimic, and GAPDH was used as an internal control. RD cells were transfected with the NC (100 nM), hsa-let-7c-5p (100 nM) or neither for 48 h, followed by EV71 infection (MOI = 5) for 24 h. In addition, RD cells were subjected to mock infection, as shown in (d), to assess specificity of the VP1 primary antibody. The lower panel in (d) shows that VP1 expression normalized to GAPDH is expressed as a fold change compared with the Mock-T group, as quantified using Image J software. e The effect of the hsa-let-7c-5p mimic on cell viability was assessed by MTT assay. RD cells were transfected with the NC (100 nM) or hsa-let-7c-5p mimic (100 nM) for 48 h, followed by EV71 infection (MOI = 5) or mock infection for 24 h. Cell viability is expressed as percentages of the NC-transfected and mock-infected cells, which were used as controls. *p < 0.05; **p < 0.01; ***p < 0.001; ns no significance

Back to article page