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Fig. 7 | Cell & Bioscience

Fig. 7

From: Identification of target genes for spermatogenic cell-specific KRAB transcription factor ZFP819 in a male germ cell line

Fig. 7

Expression analysis of Tnrc6b and Anxa11. a The transcript levels of Tnrc6b and Anxa11 were examined by qRT-PCR in mock vector- or Zfp819-transfected cells. The qRT-PCR data of Tnrc6b and Anxa11 were normalized with that of Gapdh. Experiments were repeated three times. The data are expressed as the mean ± SEM; *p < 0.05 and **p < 0.001 (Student’s t test). b, c The expression of TNRC6B in Zfp819-overexpressing cells. Expression level of TNRC6B were examined at 48 h post-transfection by Western blotting (b), and the intensities of the bands were quantified (c). The data are expressed as the mean ± SEM (n = 3); *p < 0.01. d Tissue distributions of Tnrc6b and Anxa11. Complementary DNAs from eight different mouse adult tissues and two different cell lines were subjected to RT-PCR analysis. The glyceraldehyde-3-phosphate dehydrogenase (Gapdh) gene was used as a control. e Western blotting demonstrated the expression of TNRC6B in eight different mouse adult tissues. GAPDH was used as a control

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