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Fig. 6 | Cell & Bioscience

Fig. 6

From: Identification of target genes for spermatogenic cell-specific KRAB transcription factor ZFP819 in a male germ cell line

Fig. 6

Direct regulation of Tnrc6b and Anxa11 by ZFP819. a Schematic diagrams of Tnrc6b and Anxa11 promoters. Black boxes mean sites including probes used for ChIP-chip experiment. b Western blotting of GC-2 cells at 24 h after transfection with Zfp819 or empty vector. The α-tubulin protein was used as a control. Analysis of Tnrc6b (c) and Anxa11 (d) promoters. GC-2 cells were cotransfected with each promoter-luciferase constructs and Zfp819 or empty vector. Promoter activity was evaluated in dual-luciferase assays. pGL3-promoter vector was used as a control. Experiments were conducted in triplicate. The data are expressed as the mean ± SEM; *p < 0.05, **p < 0.01 versus mock (Student’s t test)

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