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Fig. 5 | Cell & Bioscience

Fig. 5

From: Identification of target genes for spermatogenic cell-specific KRAB transcription factor ZFP819 in a male germ cell line

Fig. 5

Validation of ChIP-chip analyses. a Venn diagram representing comparison of the data between microarray and ChIP-chip in Zfp819-overexpressing GC-2 cells. b Peak graphs of Tnrc6b or Anxa11 based on log2 ratio from ChIP-chip assay. Dotted lines mean log2 ratio of Tnrc6b or Anxa11 probes from mock-transfected cells. Black lines represent log2 ratio of Tnrc6b or Anxa11 probes from Zfp819-transfected cells. c Schematic structures of Tnrc6b and Anxa11 promoter regions including probes. ChIP-PCR assay was performed with primer sets according to each probe sites (T1–T3 and A1–A3). Input was used as a control

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