Fig. 2

Behavior and apoptosis of Zfp819-overexpressing cells. Cells transfected with mock vector or Zfp819 were analyzed after 48 h. a Western blotting of GC-2 cells at 48 h after transfection with Zfp819 or empty vector. b Proliferation of Zfp819-overexpressing GC-2 cell line, as assessed by MTT assays. Experiments were repeated three times. The data are expressed as the mean ± SEM; *p < 0.001 (Student’s t test). c, d Cell cycle distribution of Zfp819-overexpressing GC-2 cells stained with propidium iodide and assessed by flow cytometry. Histograms of cell cycle distribution (c). All experiments were performed three times. The data are expressed as the mean ± SEM; *p < 0.05 and **p < 0.01 versus mock (Student’s t test). e TUNEL-positive signals (arrowheads) were observed using confocal microscopy after 48 h. f Histogram of TUNEL analysis. More than 1000 cells were counted. The experiment was repeated three times. The data are expressed as the mean ± SEM; *p < 0.001 versus mock (Student’s t test)