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Fig. 1 | Cell & Bioscience

Fig. 1

From: Identification of target genes for spermatogenic cell-specific KRAB transcription factor ZFP819 in a male germ cell line

Fig. 1

Transcriptional repression by the Zfp819 protein. a Diagram of the expression and reporter constructs used for the reporter assay. b Western blotting of GAL4-DBD-Myc and Zfp819-GAL4-DBD-Myc expressed in GC-2 cells. c GC-2 cells were transfected and subjected to reporter assays. Both KRAB-ZF genes had strong repressive activities. GAL4-DBD-Myc alone and KOX1 were used as controls. Experiments were conducted in triplicate. The data are expressed as the mean ± SEM; *p < 0.01 versus GAL4-DBD-Myc (Student’s t test)

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