Table 2 Fission yeast strains and plasmids
From: A fission yeast cell-based system for multidrug resistant HIV-1 proteases
Strains and plasmids | Genotype and characters | Source or reference |
|---|---|---|
S. pombe strains | ||
 SP223 | wild type, h − , ade6-216, leu1-32, ura4-294 | Laboratory collection |
Plasmids | ||
 pYZ1N | Fission yeast expression vector with an inducible nmt1 promoter and a LEU2 selectable marker | [50] |
 pYZ2N | Same as pYZ1N but with a ura4 selectable marker | [50] |
 pYZ3N | Same as pYZ1N but with a 5′ GFP-tag | [50] |
 pYZ1N-PR | Wild type HIV-1 PR gene cloned in pYZ1N Used to test its effect on cell growth and killing | [32] |
 pYZ1N-M7 | Drug resistant M7 HIV-1 drPR gene cloned in pYZ1N Used to test its effect on cell growth and killing | This study |
pYZ1N-M10 | Drug resistant M10 HIV-1 drPR gene cloned in pYZ1N Used to test its effect on cell growth and killing | This study |
 pYZ1N-M11 | Drug resistant M11 HIV-1 drPR gene cloned in pYZ1N Used to test its effect on cell growth and killing | This study |
 pYZ2N-PR | Wild type HIV-1 PR gene cloned in pYZ2N Used to test HIV-1 PR proteolytic cleavage | [32] |
 pYZ2N-M7 | Drug resistant M7 HIV-1 drPR gene cloned in pYZ2N Used to test HIV-1 PR proteolytic cleavage | This study |
 pYZ2N-M10 | Drug resistant M10 HIV-1 drPR gene cloned in pYZ2N Used to test HIV-1 PR proteolytic cleavage | This study |
 pYZ2N-M11 | Drug resistant M11 HIV-1 drPR gene cloned in pYZ2N Used to test HIV-1 PR proteolytic cleavage | This study |
 pYZ3N-GFP-MA-Vpr | GFP is connected to Vpr in pYZ3N by a polylinker containing the PR cleavage MA↓CA site (DSQNY↓PIVQ) | [32] |
 pYZ3N-p6-MA-Vpr | GFP is connected to Vpr in pYZ3N by a polylinker containing the PR cleavage p6 site (DSFNF↓PQIT) | [32] |