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Table 2 Fission yeast strains and plasmids

From: A fission yeast cell-based system for multidrug resistant HIV-1 proteases

Strains and plasmids Genotype and characters Source or reference
S. pombe strains
 SP223 wild type, h , ade6-216, leu1-32, ura4-294 Laboratory collection
Plasmids
 pYZ1N Fission yeast expression vector with an inducible nmt1 promoter and a LEU2 selectable marker [50]
 pYZ2N Same as pYZ1N but with a ura4 selectable marker [50]
 pYZ3N Same as pYZ1N but with a 5′ GFP-tag [50]
 pYZ1N-PR Wild type HIV-1 PR gene cloned in pYZ1N
Used to test its effect on cell growth and killing
[32]
 pYZ1N-M7 Drug resistant M7 HIV-1 drPR gene cloned in pYZ1N
Used to test its effect on cell growth and killing
This study
pYZ1N-M10 Drug resistant M10 HIV-1 drPR gene cloned in pYZ1N
Used to test its effect on cell growth and killing
This study
 pYZ1N-M11 Drug resistant M11 HIV-1 drPR gene cloned in pYZ1N
Used to test its effect on cell growth and killing
This study
 pYZ2N-PR Wild type HIV-1 PR gene cloned in pYZ2N
Used to test HIV-1 PR proteolytic cleavage
[32]
 pYZ2N-M7 Drug resistant M7 HIV-1 drPR gene cloned in pYZ2N
Used to test HIV-1 PR proteolytic cleavage
This study
 pYZ2N-M10 Drug resistant M10 HIV-1 drPR gene cloned in pYZ2N
Used to test HIV-1 PR proteolytic cleavage
This study
 pYZ2N-M11 Drug resistant M11 HIV-1 drPR gene cloned in pYZ2N
Used to test HIV-1 PR proteolytic cleavage
This study
 pYZ3N-GFP-MA-Vpr GFP is connected to Vpr in pYZ3N by a polylinker containing the PR cleavage MA↓CA site (DSQNY↓PIVQ) [32]
 pYZ3N-p6-MA-Vpr GFP is connected to Vpr in pYZ3N by a polylinker containing the PR cleavage p6 site (DSFNF↓PQIT) [32]