Fig. 2

Production of HIV-1 _mdrPRs prevent yeast colony formation, cell growth and lead to cell death. A The inducible expression of the _wt PR and _mdr PR genes in fission yeast 24 h after gene induction produced similar levels of HIV-1 PR proteins as detected by the western blot analysis (a), prevented the yeast colony formation (b) and the cellular growth over time (c). Note that the IDV treatment only prevented the effect of the _wtPR but not the _mdrPRs. The IDV concentration was added in (b) as shown. 100 µg/ml of IDV was added to the _mdr PR-expressing cells in (c). All cells were grown at 30 °C and the cell growth was measured by OD₆₅₀ in the time period as indicated by using spectrophotometer. B Both the _wtPR and _mdrPR-induced cell death are shown by a yeast live/dead assay [27, 32]. Note that adding IDV before the gene induction only prevented the cell death induced by the _wtPR (upper row) but not that by the _mdrPR. Pictures were taken at 24 h after the gene induction. IDV (−), i.e., no IDV added; IDV (+), 100 µg/ml of IDV was added prior to the gene inductions