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Fig. 4 | Cell & Bioscience

Fig. 4

From: Characterization of tubular liquid crystal structure in embryonic stem cell derived embryoid bodies

Fig. 4

Time-lapse recordings of temperature dependent phase transition of embryoid body birefringent liquid crystals. Birefringence of liquid crystals distributed in the embryoid body is lost as the crystals assume isotropic status with temperature increase to 43 °C (af). There is incomplete recovery of the birefringent structure as the thermostage resumed room temperature (h, i). This change in birefringence following temperature change and time progression is quantifiable (j). There are no significant differences among birefringence density of T1–T3, but extremely significant difference in T4–T13 based on references T1. Magnifications of the liquid–crystal structures before and after temperature transition highlight the incomplete resumption of the original structure (k, l, respectively). The decrease in size of EB liquid crystal structures is quantified by the significantly smaller diameters Maltese’s cross (D1 before and D1 after, p = 0.000003; D2 before and D2 after, p = 0.000001) and tubular structures (W1 before and W1 after, p = 0.000083; W2 before and W1 after, p = 0.003474) is quantified (M). *p < 0.05 and **p < 0.01 in j. Scale bars are 300 μm in (ai); 100 μm in (k, l)

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