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Fig. 5 | Cell & Bioscience

Fig. 5

From: Small GTPase Arl6 controls RH30 rhabdomyosarcoma cell growth through ciliogenesis and Hedgehog signaling

Fig. 5

Knockdown of Arl6 induced cell cycle arrest and apoptosis of RH30 cells. a FACS analysis of the cell cycle profile in RH30 cells stably expressed shArl6 or nonsense control. 10,000 cells were analyzed in each sample. Quantitation of cell cycle distribution of RH30 cells from experiment (a) was shown in b. For apoptosis assays (c), the cells were maintained in DMEM supplemented with 0.2% FBS and processed for apoptosis assay 2 days later by Annexin V/PI double staining and FACS analysis. d Apoptotic rate was shown as the percentage of Annexin V + cells. The data represents the mean ± SEM of three independent experiments *P < 0.05, **P < 0.01 vs. shCon

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