Fig. 4

Knockdown of Arl6 suppresses cell growth of RH30. MTT assays for RH30 (a) and RD (b) cells stably expressing shArl6 or nonsense control. Q-PCR detection of Gli1 mRNA levels in RH30 (c) and RD (e) stable cells. The knockdown efficiency of shArl6 in RH30 (d) and RD (f) cells were also detected by Q-PCR. Q-PCR detection of Hh target genes Gli2, Hhip and Ptch1 mRNA levels in RH30 (g, h, i) and RD (j, k, l) stable cells. Q-PCR detection of Hh ligands Dhh and Ihh mRNA levels in RH30 (m, n) and RD (o, p) stable cells. q Representative fluorescence images and r percentage quantification of EdU incorporation assays of RH30 and RD stable cells. The cells were assayed in 24-well plates. The data was obtained in triplicate. Data in a–p and r are presented as mean ± SEM of three independent experiments. The error bars denote SEM. *P < 0.05 and **P < 0.01 vs. shCon