Fig. 4

miR-19b targets Sox6 gene and inhibits its expression. a The sketch map of the mmu-miR-19b-3p site in Sox6 3′-UTR. b Luciferase activity assay was performed in 293T cells at 48 h after transfection. The reporter assay indicated that miR-19b mimics were capable of significantly inhibiting luciferase expression of the Sox6-WT reporter plasmid but not the Sox6-Mut reporter plasmid. c Western blot showed that protein level of Sox6 was low from day 0 to 6, and was significantly upregulated from day 8 to 12. d The Sox6 protein levels were significantly down- and up-regulated respectively in P19CL6 cells transfected with miR-19b and anti-miR-19b at day 10. e shH19 inhibited the Sox6 expression significantly, but miR-19b enhanced the inhibitory effect of shH19 on Sox6 expression and anti-miR-19b reversed the influence of shH19 on Sox6 expression at day 10 after transfection. *P < 0.05, **P < 0.01, ***P < 0.001