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Fig. 2 | Cell & Bioscience

Fig. 2

From: Downregulation of long non-coding RNA H19 promotes P19CL6 cells proliferation and inhibits apoptosis during late-stage cardiac differentiation via miR-19b-modulated Sox6

Fig. 2

The effect of H19 knockdown and H19 overexpression on P19CL6 cell proliferation and apoptosis. a Transfecting P19CL6 cells with shH19 and pcDNA-H19 efficiently down- and up-regulated the H19 level at day 10, respectively. b CCK-8 assay showed that shH19 resulted in an increase in cell viability at day 8 and 10, whereas pcDNA-H19 had an opposite effect. c Flow cytometry suggested that shH19 significantly increased the cell number in S phase at day 8 and 10, and pcDNA-H19 significantly decreased the cell number. d shH19 significantly reduced the apoptotic rate of P19CL6 cells at day 8 and day 10, whereas pcDNA-H19 had an opposite effect. e Caspase-3 assay indicated that the activity of caspase-3 was significantly reduced in P19CL6 cells transfected with shH19 and significantly increased in cells transfected with pcDNA-H19. *P < 0.05, **P < 0.01, ***P < 0.001

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