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Fig. 2 | Cell & Bioscience

Fig. 2

From: One-step immortalization of primary human airway epithelial cells capable of oncogenic transformation

Fig. 2

Characterization of the proliferation and morphology of iSAECs. a Population doubling (PD) curves for SAECs transduced with indicated cDNA and shRNA pairs. Only SAECs transduced with hTERT cDNA and sh_p16 were able to proliferate indefinitely. b Morphology of early and late passages (P19 and P71, respectively) iSAEC cells. c Expression of the epithelial marker keratin 19 (KRT19) in iSAECs. BJ fibroblasts and the NSCLC cell line HCC4006 were used as negative and positive controls, respectively. KRT19 mRNA levels were normalized to beta-actin. d Expression of E-cadherin and vimentin in iSAECs. BJ fibroblasts and the NSCLC cell line HCC4006 were used as controls

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