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Fig. 1 | Cell & Bioscience

Fig. 1

From: One-step immortalization of primary human airway epithelial cells capable of oncogenic transformation

Fig. 1

Characterization of retroviral vector for one-step immortalization. a Schematics of the MSCV-pic2 vector design. For one-step immortalization, a hTERT cDNA and a shRNA targeting p16INK4A and p14ARF were introduced into this vector. b Western blot verifying the knockdown of p16INK4A and p14ARF in stably transduced SAECs. c Telomerase activities in SAECs stably transduced with hTERT cDNA (error bars represent SD for all bar charts unless otherwise stated)

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