Fig. 5

The effects of different treatments on activation of PI3K/Akt and expression of VEGF. Western blotting of total cell lysates treated with the indicated reagents for 1 h was performed, and β-actin was used as a loading PBS control. A The levels of PI3K and Akt phosphorylation were determined by phosphorylated proteins standardized to non-phosphorylated proteins. B The VEGF level of the cell lysates was determined by standardizing VEGF expression to β-actin expression. The results represent the mean ± SD values from three independent experiments. *p < 0.05, **p < 0.01 vs. the PBS control; ^##p < 0.01 vs. 1.0 µM roxarsone