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Fig. 4 | Cell & Bioscience

Fig. 4

From: Roxarsone induces angiogenesis via PI3K/Akt signaling

Fig. 4

The effects of different treatments on endothelial cell (EC) tube formation. A After treatment with the indicated reagents for 6 h, tubular structure in each group was photographed at ×200 magnification. Representative images are shown for the a PBS control; b VEGF; ce 0.1, 1.0 and 10.0 µM roxarsone, f LY294002 and g LY294002 plus 1.0 µM roxarsone groups. B The number of meshes in five randomly selected images for each group was measured using Image-J with the Angiogenesis Analysis Plugin. The results represent the mean ± SD values from three independent experiments. *p < 0.05, **p < 0.01 vs. the PBS control; ##p < 0.01 vs. 1.0 µM roxarsone

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