Fig. 3

The effects of different treatments on the migration ability of endothelial cells (ECs). A ECs were wounded with a pipette and treated with the indicated reagents for 24 h. Representative images (×100) are shown for the a PBS control; b VEGF; c–e 0.1, 1.0 and 10.0 µM roxarsone, f LY294002 and g LY294002 plus 1.0 µM roxarsone groups. B The distance that the cells had migrated was calculated by subtracting the width of the scratch at 0 h from the width at 24 h. The results represent the mean ± SD values from three independent experiments. *p < 0.05, **p < 0.01 vs. the PBS control; ^##p < 0.01 vs. 1.0 µM roxarsone