Fig. 4
GCN5 knockdown inhibits cell cycle progression. a Cell cycle analysis of stable GCN5-knockdown cells and control cells were performed by flow cytometry. A total of 4 × 105 cells were seeded into six-well plates, synchronized by serum starvation for 24 h and re-entered into the cell cycle by an exchange of 10 % FBS DMEM for 24 h. Cell were harvested and cell cycle status was measured by flow cytometry. **p < 0.01. b The protein levels of PCNA, p-Akt and AIB1 were decreased, but the protein levels of p21 were increased in GCN5-knockdown cells. c The mRNA levels of AIB1 were decreased, but the mRNA levels of p21 were drastically increased in GCN5-knockdown cells. *p < 0.05, **p < 0.01. d Forced expression of AIB1 rescued the proliferation of GCN5-knockdown HepG2 cells. Each experiment was performed at least twice with similar results. All data are the means + sd. (n = 3) at each time point. *p < 0.05, **p < 0.01. e Forced expression of AIB1 in GCN5 knockdown cells decreased the protein level of p21