Fig. 2

Effect of E2 in hiPSC-derived hematopoiesis. a, b Effects of E2 on the production efficiency of hematopoietic progenitors (CD34⁺CD45⁺) and mature hematopoietic cells (CD34⁻CD45⁺) from hiPSC-derived EBs at day 15. EBs were cultured in hematopoietic induction medium alone or supplemented with different combination of E2, hGFs and ICI 182,780. c Assessment of hematopoietic output by restricting the period of E2 exposure during EB differentiation to phase I (I) and phase II (II) alone compared to differentiation during both phase I and II (I+II). d Assessment of hematopoietic progenitor capacity of EBs differentiated under various treatments for 15 days. e Distribution of CFU types (CFU-E, erythroid; CFU-M, macrophage; and CFU-G, granulocytes). All results are mean ± SD. *p < 0.05, **p < 0.01