Fig. 5

Blocked intracellular transport in Otg1 knockdown cells. GH3 cells were co-transfected with VSVG-mEmerald and Otg1 shRNA or scramble shRNA. a Scatter plot showing decreased transport speed of VSVG-mEmerald vesicles in Otg1 knockdown (red triangles) and scramble shRNA treated (black dots) GH3 cells. Eleven cells were scored for each group. Ten vesicles were randomly picked from each cell. b Scatter plot showing Otg1 knockdown resulted in decreased standard deviation of transport speed (std speed) and unaltered directionality of VSVG-mEmerald vesicles in each cell. Data of vesicles selected in (a) were used for the calculation. c, d A representative image (c) and its close-up (d) showing tracked movement of 10 VSVG-mEmerald vesicles (lines in color) in scramble shRNA treated GH3 cells. (e, f) A representative image (e) and its close-up (f) showing tracked movement of 10 VSVG-mEmerald vesicles (lines in color) in Otg1 knock down GH3 cells. Scale bars 5 μm. *p < 0.05, **p < 0.01, ***p < 0.005