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Table 1 Primers used in this study

From: The cathepsin S cysteine proteinase of the burrowing nematode Radopholus similis is essential for the reproduction and invasion

Primer name

Sequence

Primer use

Rs-cps-S1

5′-AGTGCCCCTCCGAAATGT-3′

RT-PCR

Rs-cps-A1

5′-TGTCCGTTCTTCCGTTCA-3′

SF

5′-TGTTGGCGGTCCCTGTG-3′

Southern blot

SR

5′-CGTGTTCGTGGACGGAGTT-3′

 

Ish-T7S1a

5′-GGATCCTAATACGACTCACTATAGGGTGTTGGCGGTCCCTGTG-3′

ISH template

Ish-A1

5′- CCGTTCTCCTCGATGTAGTCA -3′

Ish-S2

5′-TGTTGGCGGTCCCTGTG-3′

ISH template

Ish-T7A2a

5′-GGATCCTAATACGACTCACTATAGGGCCGTTCTCCTCGATGTAGTCA-3′

qPCR-F

5′-AGAACTCCGTCCACGAACAC-3′

qPCR

qPCR-A

5′-GCCCACATTGCGCTTGCT-3′

Actin-F

5′-GAAAGAGGGCCGGAAGAG-3′

qPCR

Actin-R

5′-AGATCGTCCGCGACATAAAG-3′

CPS-T7Sa

5′-GGATCCTAATACGACTCACTATAGGGTGTTGGCGGTCCCTGTG-3′

dsRNA template

CPS-A

5′- CGTGTTCGTGGACGGAGTT-3′

CPS-S

5′-TGTTGGCGGTCCCTGTG-3′

dsRNA template

CPS-T7Aa

5′-GGATCCTAATACGACTCACTATAGGG CGTGTTCGTGGACGGAGTT-3′

eGFP-T7Sa

5′-GGATCCTAATACGACTCACTATAGGGCAGTGCTTCAGCCGCTACC-3′

dsRNA template

eGFP-A

5′-AGTTCACCTTGATGCCGTTCTT-3′

eGFP-S

5′-CAGTGCTTCAGCCGCTACC-3′

dsRNA template

eGFP-T7 Aa

5′-GGATCCTAATACGACTCACTATAGGGAGTTCACCTTGATGCCGTTCTT-3′

  1. aThe T7 promoter sequence is italiced