Fig. 5
ESE-1 knockout (KO) bone marrow derived macrophages (BMDMs) show increased COX-2 expression and an increased propensity towards M1 phenotype. a Expression of macrophage maturation markers CD11b and F4/80 in BMDMs derived from WT or Elf3 KO C57BL/6 mice. Bone marrow cells isolated from the WT or Elf3 mice were differentiated ex vivo in 20 % L-929-conditioned media and analyzed by flow cytometry. The graph shows n = 12 for WT and KO mice. b ESE-1 knockout BMDMs show increased COX-2 mRNA following LPS (100 ng/mL) treatment. BMDMs were plated at 4 x 105/well on 6-well plates and stimulated with 100 ng/mL LPS or 10 ng/mL IL-4 for 18 h, after which they were lysed for RNA isolation and qPCR analysis (n = 6). c shows mRNA expression of genes related to M1 polarization and d M2-related genes in WT and KO BMDMs (n = 6) treated with 100 ng/mL LPS (“M1”) and 10 ng/mL IL-4 (“M2) for 18 h prior to analysis. Statistical analysis by one-way ANOVA with Bonferroni’s post-test for selected pairs, ns = not significant, *P < 0.05, **P < 0.01