Fig. 4

ESE-1 downregulation leads to increased COX-2 mRNA in SW982 cell line. a Analysis of ESE-1, COX-2, RelA and NFκB1 transcriptional levels by RT-qPCR in SW982 cells during IL-1β (10 ng/mL) stimulation. b Representative Western blot of SW982 stimulated with IL-1β (10 ng/mL) over the 24 h time period. c Time course analysis of SW982 cells transduced with C4HSU or shESE-1 HD-Ad vectors and stimulated with IL-1β over 24 h, started 72 h post-transduction. *P < 0.05, **P < 0.01, ***P < 0.001, by one-way ANOVA Bonferroni’s post-test, data shown are representative of two independent experiments with n = 3 each