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Fig. 3 | Cell & Bioscience

Fig. 3

From: Epithelium-specific Ets transcription factor-1 acts as a negative regulator of cyclooxygenase-2 in human rheumatoid arthritis synovial fibroblasts

Fig. 3

ESE-1 is expressed in the nucleus of RASFs. a ESE-1 protein level increases with IL-1β stimulation (n = 4), shown by Western blot on whole cell lysates (WCLs) of stimulated or unstimulated RASFs. #1–#4 denotes patients #50, 58, 77, and 22, and A549 lysate was included as positive control for ESE-1 protein. b ESE-1 protein is exclusively expressed in the nucleus, with Hsp90 and Lamin A as cytoplasmic and nuclear markers, respectively. Western blot of nuclear fractionated RASFs stimulated with 10 ng/mL IL-1β for 24 h. c ESE-1 and COX-2 are minimally present in RASFs without IL-1β stimulation. d Knockdown of ESE-1 by HD-Ad-shESE-1 leads to increase in COX-2 expression in the cytoplasm in IL-1β treatment in RASFs compared to C4HSU control vector. Western blot showing results from two different patient RASFs

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