Fig. 2
Knockdown of ESE-1 leads to increased COX-2 and PGE2 production in RASF. a Representative picture of human RASFs successfully transduced with helper-dependent adenovirus containing EGFP construct (HD-Ad-EGFP) with and without DEAE-Dextran. b Knockdown of ESE-1 leads to the transcriptional upregulation of COX-2 following 24 h of IL-1β stimulation (n = 6). The cells were incubated in the presence of HD-Ad and DEAE-Dextran complex for 48 h and starved in 0.5 % FBS DMEM for 24 h prior to incubation with IL-1 β. Scale bar shows 100 μm. c Transcriptional upregulation of COX-2 is accompanied by increased production of PGE2 (n = 6). d Representative Western blot showing changes in ESE-1 and COX-2 proteins in RASFs transduced with control (C4HSU) or shESE-1 HD-Ad. Bars show the mean ± SEM, *P < 0.05, **P < 0.01, ***P < 0.001, by one-way ANOVA Bonferroni’s post-test. (E) Time point analysis of ESE-1 and COX-2 transcriptional levels (n = 5) during IL-1β stimulation after C4HSU or shESE-1 HD-Ad viral transduction. ns not significant, *P < 0.05, ***P < 0.001