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Fig. 5 | Cell & Bioscience

Fig. 5

From: Hedgehog pathway is involved in nitidine chloride induced inhibition of epithelial-mesenchymal transition and cancer stem cells-like properties in breast cancer cells

Fig. 5

NC induced inhibition of in breast cells by Hedgehog-Gli1 pathway. After both cells were pretreated by cyclopamine (a Smo inhibitor) for 6 h, they were exposed to 0, 1, or 2.5 μM of NC for 48 h respectively. a Transwell assay analyses of the migratory capacity of both cells. Then quantification of transwell cell migration assay. b Free-floating, viable spheres formed by breast cancer cells. c Western blotting analyses of Gli1, Smo, E-cadherin, N-cadherin, Vimentin, CD44 levels. Blots were normalized by use of β-actin to correct for differences in loading of the proteins.*P < 0.05 or **P < 0.01. The data are presented the mean ± SD of three separate experiments

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