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Fig. 4 | Cell & Bioscience

Fig. 4

From: Potential role of oxidative stress-induced apoptosis in mediating chromosomal rearrangements in nasopharyngeal carcinoma

Fig. 4

H2O2 induces AF9 cleavages in HK1 cells that are abolished by caspase inhibitor. (a) Representative gel pictures showing the IPCR analysis of H2O2-treated HK1 cells: (i) without CI pre-treatment (ii) with CI pre-treatment. HK1 cells were left untreated or pre-treated with 50 µM of Z-DEVD-FMK (Caspase-3 Inhibitor II) for 1 h. The cells were then either untreated (Lanes 16) or treated with 50 µM of H2O2 for 8 h (Lanes 712). Genomic DNA was extracted and modified for nested IPCR as described in “Methods” section. Six replicates (R1–R6) were prepared for each cell sample in nested IPCR. The IPCR bands derived from the AF9 cleaved chromosome were indicated by the side brackets. N: Negative control for IPCR. M: 100 bp DNA ladder. (b) AF9 cleavage frequency detected in HK1 cells. Data are representative of two independent experiments. Each experiment consisted of 6 IPCR replicates for each cell sample. The results are presented as medians with IQRs. *P < 0.05 (Mann–Whitney U test)

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