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Fig. 1 | Cell & Bioscience

Fig. 1

From: Potential role of oxidative stress-induced apoptosis in mediating chromosomal rearrangements in nasopharyngeal carcinoma

Fig. 1

H2O2 induces PS externalization in HK1 and NP69 cells. HK1 cells were either left untreated or treated with 50 µM of H2O2 for 4 and 8 h, whereas NP69 cells were either left untreated or treated with 100 µM of H2O2 for 16 and 24 h. The cells were then subjected to flow cytometric analysis of PS externalization as described in “Methods” section. Cells treated with CPT were included as a positive control. Percentages of apoptotic cells expressing PS were determined in (a) (i) HK1 and (b) (i) NP69 upon H2O2 treatment. Means and SD from three independent experiments carried out in duplicate are shown. Student’s t test was used for statistical analysis to compare treated groups with untreated control, *p < 0.05. Representative dot plot diagrams showing the apoptotic populations of (a) (ii) H2O2-treated HK1 cells and (b) (ii) H2O2-treated NP69 cells detected by Annexin V-FITC and PI staining are shown. The lower left quadrants show viable cells; the lower right quadrants represent early apoptotic cells; the upper right quadrants show late apoptotic and necrotic cells

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