Table 2 Potential methods for HCC ctDNA detection
From: Circulating tumor DNA in hepatocellular carcinoma: trends and challenges
Technique | Detection capability (mutant DNA/total DNA) (%) |
|---|---|
Sanger sequencing | >10 |
Pyrosequencing | 10 |
Next-generation sequencing | >1 |
Real-time | 1 |
ARMS | 0.1 |
Digital droplet PCR, BEAMing, PAP, TAM-seq, Safe-SeqS, CAPP-seq, PARE | <0.01 |