Fig. 5

Distinct roles of tyrosine 625 and 630 in MPL W515L-induced MPNs. a Mice (n = 3) were transplanted with bone marrow progenitor cells transduced with various MPL constructs as indicated. The engraftment of the transduced cells (GFP⁺) in the peripheral blood was monitored by flow cytometry over time as indicated. b The white blood counts and the platelet counts in the peripheral blood of the recipient mice were presented. c The recipient mice were sacrificed 1 month after transplantation. The spleen and liver weight was measured. d Representative photos of the histopathology of sternum, spleen, liver and lung from recipient mice were presented. Arrows highlight the megakaryocytes in the H&E sections. *^, ** or *** indicates significance (p < 0.05, 0.01, 0.001, respectively) and NS indicates non-significance compared to MPL W515L