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Fig. 3 | Cell & Bioscience

Fig. 3

From: Tyrosine 625 plays a key role and cooperates with tyrosine 630 in MPL W515L-induced signaling and myeloproliferative neoplasms

Fig. 3

STAT5-deficiency impairs MPL W515L-induced megakaryocyte hyperproliferation. a Bone marrow cells from F/− or F/+ mice treated with poly I-C were used for western blot to detect the total STAT5 (STAT5A and STAT5B). Hsc70 was the loading control. b F/+ and F/− bone marrow progenitor cells were transduced with control retrovirus (Ctrl) or MPL W515L-expressing virus. The resultant cells were purified and used for CFU-Mk. c The transduced cells were also cultured for megakaryocyte differentiation to detect CD41 expression by flow cytometry. d The DNA content in CD41+ cells was also measured by staining cells with DAPI. Numbers indicate the percentage of gated cells. Cells with DNA > 4N were polyploid cells for statistics. All bar graphs are the statistics of one representative experiment (duplicate) from two independent experiments with similar results. Asterisk indicates significance (p < 0.05) and NS indicates non-significance

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