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Fig. 1 | Cell & Bioscience

Fig. 1

From: Tyrosine 625 plays a key role and cooperates with tyrosine 630 in MPL W515L-induced signaling and myeloproliferative neoplasms

Fig. 1

Tyrosine 625 is critical for MPL W515L-induced TPO-independent cell proliferation and megakaryocyte hyperproliferation. a Diagram illustrates the wild-type MPL and four MPL mutants. Stars and numbers indicate mutation sites. b G1ME cells transduced with each construct were cultured without TPO. The cell numbers were counted over time and normalized to the starting cell numbers (Day 0) as proliferation rate. c WT bone marrow progenitor cells transduced with each construct were used for CFU-Mk assay. The bar graphs are the statistics of one representative experiment with duplicates from two independent experiments with similar results. Asterisk indicates significance (p < 0.05) and NS indicates non-significance compared to MPL W515L. d Cells were cultured in the absence of TPO and harvested for western blot to detect the phosphorylation and the expression level of each protein as indicated. Hsc70 is the loading control

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