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Table 2 Sequences of primers used to amplify and directly sequence exons 1, 2, 4 of the human HNF-1α gene

From: Studies of genetic variability of the hepatocyte nuclear factor-1α gene in an Indian maturity-onset diabetes of the young family

Exon

Sense primer (5′–3′)

Antisense primer (5′–3′)

Tm (°C)

Product size (bp)

1

GGCAGGCAAACGCAACCCACG

GAAGGGGGGCTCGTTAGGAGC

60

483

2

CATGCACAGTCCCCACCCTCA

CTTCCAGCCCCCACCTATGAG

58

390

4

CAGAACCCTCCCCTTCATGCC

GGTGACTGCTGTCAATGGGAC

62

397