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Fig. 5 | Cell & Bioscience

Fig. 5

From: SP1 and UTE1 Decoy ODNs inhibit activation and proliferation of hepatic stellate cells by targeting tissue inhibitors of metalloproteinase 1

Fig. 5

Influnce of SP1 and UTE1 Decoy ODNs on TGF-β and TRE in HSC-T6 cells. a Influnce of SP1 and UTE1 Decoy ODNs on the expression of TGF-β by western blot assays in HSC-T6 cells. The β-actin protein served as control. D-SP1 SP1 Decoy ODN, D-UTE1 UTE1 Decoy ODN, Scramble Scr Decoy ODN. b Quantification of TGF-β expression in HSC-T6 cells by western blot did not show significant changes (p > 0.05) among the groups. Data are presented as the mean ± SD of three experiments. The band intensities were normalized to β-actin in the quantificative analysis. c Influence of SP1 and UTE1 Decoy ODNs on the activity of TRE in HSC-T6 cells. After pGLuc-TRE-MiniTK was transfected into HSC-T6 cells for 24 h, Decoy ODNs were transfected for another 24 h. Data are presented as the mean ± SD of three experiments and each experiment for three wells. ΔΔp < 0.01 represented three experimental groups compared to Scr Decoy ODN group respectively. **p < 0.01

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