Fig. 4

The expression of COLIα2 and α-SMA dealed with SP1 and UTE1 Decoy ODNs by Western blot assay. D-SP1 SP1 Decoy ODN, D-UTE1 UTE1 Decoy ODN, Scramble Scr Decoy ODN. Data are presented as the mean ± SD of three experiments. ^Δp < 0.05 and ^ΔΔp < 0.01 represented three experimental groups compared to Scr Decoy ODN group respectively. *p < 0.05 and **p < 0.01. The β-actin protein served as control and band intensities were normalized to β-actin in the quantificative analysis. a The expression of COLIα2 and α-SMA was analysed by western blot assays when SP1 and UTE1 Decoy ODNs were transfected into HSC-T6 cells for 48 h. b Quantification of COLIα2 expression in HSC-T6 cells by western blot showed a significant decrease (p = 0.034) in average band intensity with group of SP1 Decoy ODN, however, there was not obvious difference from group of UTE1 Decoy ODN (p = 0.220), compared to scramble control, respectively. There are significant decreases comparing group of combinational treatment of SP1 and UTE1 Decoy ODNs, not only to scramble control (p = 0.001), but also with group of SP1 Decoy ODN (p = 0.044) or group of UTE1 Decoy ODN (p = 0.030), respectively. c Quantification of α-SMA expression in HSC-T6 cells by western blot showed obvious decreases (p = 0.020; p = 0.010; p = 0.000) in average band intensity with three experimental groups (SP1 Decoy ODN group, UTE1 Decoy ODN group, mixture group of SP1 and UTE1 Decoy ODNs) compared with scramble control, respectively. There was a significant decrease (p = 0.045) comparing mixture group of SP1 and UTE1 Decoy ODNs with SP1 Decoy ODN group, however, there was no obvious difference (p = 0.179) compared to UTE1 Decoy ODN group, respectively